Background
Bcl-2 and Bcl-XL has been known to be members of Bcl-2 family for regulation of long-term cell survival.We investigated the expression of Bcl-2 and Bcl-XL in myblast during myogenesis.Methods: Cultured myoblasts from the C2C12 mouse cell line were isolated at the different stages of myoblast andmyotubes. The mRNA levels for Bcl-2 and Bcl-XL were determined by reverse-transcriptase PCR (RT-PCR). Theimmunocytochemical staining using the antibodies for Bcl-2 and Bcl-XL was performed to examine the expression ofBcl-2 and Bcl-XL in cultured C2C12 cells.Results: By immunocytochemistry, Bcl-2 was strong expressed in cultured myoblasts compared with myotubes. Theexpression of Bcl-XL were observed at similar degree in both myoblasts and myotubes. The RT-PCR assay showed thatC2C12 cells expressed both Bcl-2 and Bcl-XL mRNA level with different pattern of expression at differential stage. Thelevel of expression of Bcl-2 mRNA was observed most highly at early stage of myoblasts, but diminished as differentiationprogressed. However, the level of expression of Bcl-XL didn
