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Ann Clin Neurophysiol > Volume 6(2); 2004 > Article
Ann Clin Neurophysiol. 2004; 6(2): 80-84.
The Expression of Bcl-2 and Bcl-XL in Myoblast During Myogenesis
Seung-Hun Oh, Won-Joo Kim, and Young-Chul Choi
Copyright © 2004 The Korean Society of Clinical Neurophysiology
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
ABSTRACT
Background: Bcl-2 and Bcl-XL has been known to be members of Bcl-2 family for regulation of long-term cell survival.We investigated the expression of Bcl-2 and Bcl-XL in myblast during myogenesis.
Methods: Cultured myoblasts from the C2C12 mouse cell line were isolated at the different stages of myoblast andmyotubes. The mRNA levels for Bcl-2 and Bcl-XL were determined by reverse-transcriptase PCR (RT-PCR). Theimmunocytochemical staining using the antibodies for Bcl-2 and Bcl-XL was performed to examine the expression ofBcl-2 and Bcl-XL in cultured C2C12 cells.
Results: By immunocytochemistry, Bcl-2 was strong expressed in cultured myoblasts compared with myotubes. Theexpression of Bcl-XL were observed at similar degree in both myoblasts and myotubes. The RT-PCR assay showed thatC2C12 cells expressed both Bcl-2 and Bcl-XL mRNA level with different pattern of expression at differential stage. Thelevel of expression of Bcl-2 mRNA was observed most highly at early stage of myoblasts, but diminished as differentiationprogressed. However, the level of expression of Bcl-XL didn
Key words: Myogenesis, Apoptosis, Bcl-2, Bcl-XL, C2C12, Immunocytochemistry
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